AUTHOR=Zhou Xinli , Jia Guoyun , Luo Yuqi , Li Xin , Cai Lin , Chen Xianming , Kang Zhensheng 

TITLE=Fine mapping of QYrsv.swust-1BL for resistance to stripe rust in durum wheat Svevo

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1395223

DOI=10.3389/fpls.2024.1395223

ISSN=1664-462X

ABSTRACT=<p>Stripe rust, caused by <italic>Puccinia striiformis</italic> f. sp. <italic>tritici</italic> (<italic>Pst</italic>), is a serious disease that affects wheat worldwide. There is a great need to develop cultivars with combinations of all-stage resistance (ASR) and adult-plant resistance (APR) genes for sustainable control of the disease. <italic>QYrsv.swust-1BL</italic> in the Italian durum wheat (<italic>Triticum turgidum</italic> ssp. <italic>durum</italic>) cultivar Svevo is effective against <italic>Pst</italic> races in China and Israel, and the gene has been previously mapped to the long arm of chromosome 1B. The gene is flanked by SNP (single nucleotide polymorphism) markers <italic>IWB5732</italic> and <italic>IWB4839</italic> (0.75 cM). In the present study, we used high-density 660K SNP array genotyping and the phenotypes of 137 recombinant inbred lines (RILs) to fine map the <italic>QYrsv.swust-1BL</italic> locus within a 1.066 Mb region in durum wheat Svevo (RefSeq Rel. 1.0) on chromosome arm 1BL. The identified 1.066 Mb region overlaps with a previously described map of <italic>Yr29/QYr.ucw-1BL</italic>, a stripe rust APR gene. Twenty-five candidate genes for <italic>QYrsv.swut-1BL</italic> were identified through comparing polymorphic genes within the 1.066 Mb region in the resistant cultivar. SNP markers were selected and converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers. Five KASP markers based on SNP were validated in a F<sub>2</sub> and F<sub>2:3</sub> breeding population, providing further compelling evidence for the significant effects of <italic>QYrsv.swut-1BL</italic>. These markers should be useful in marker-assisted selection for incorporating <italic>Yr29/QYrsv.swust-1BL</italic> into new durum and common wheat cultivars for resistance to stripe rust.</p>