Raed Elferjani ¹ Shankar Pahari ¹ Raju Soolanayakanahally ^1* Krista Thompson ¹ Eiji Nambara ²

• ¹ Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Saskatoon, Saskatchewan, Canada
• ² Department of Cell and Systems Biology, Faculty of Medicine, University of Toronto, Toronto, Canada

Drought conditions severely curtail the ability of plants to accumulate biomass due to the closure of stomata and the decrease of photosynthetic assimilation rate. Additionally, there is a shift in the plant's metabolic processes towards the production of metabolites that offer protection and aid in osmoadaptation, as opposed to those required for development and growth. To limit water loss via non-stomatal transpiration, plants adjust the load and composition of cuticle waxes, which act as an additional barrier. This study investigates the impact of soil water deficit on stomatal and epicuticular water losses, as well as metabolic adjustments in two canola (Brassica napus L.) cultivars, one drought-tolerant and the other drought-sensitive. Specifically, we examined the effect of a drought, which involved reducing water holding capacity to 40%, on the levels of cysteine, sucrose, and abscisic acid (ABA) in the leaves of both cultivars. Next, we looked for potential differences in night, pre-dawn, and early morning transpiration rates and the epicuticular wax load and composition in response to drought. A substantial rise in leaf cysteine was observed in both canola cultivars in response to drought, and a strong correlation was found between cysteine, ABA, and stomatal conductance, indicating that cysteine and sulfur may play a role in controlling stomatal movement during stress. Attributes related to CO2 diffusion and photosynthetic capacity were different between the two canola cultivars, suggesting better management of water relations under stress by the droughttolerant cultivar. Epicuticular waxes were found to adjust in response to drought, acting as an additional barrier against water loss. Surprisingly, both canola cultivars responded similarly to cysteine, sucrose, and ABA) and epicuticular waxes, indicating that they were not reliable stress markers in our test setup. However, the higher level of phenylalanine in the droughttolerant canola cultivar is suggestive that this amino acid is important for adaptation to drier climates. Furthermore, a multi-trait genotype ideotype distance index (MGIDI) revealed the likely role of aspartic acid in sustaining nitrogen and carbon for immediate photosynthetic resumption after drought episodes. In conclusion, leveraging amino acid knowledge in agriculture can enhance crop yield and bolster resistance to environmental challenges.