AUTHOR=Luo Xianyang , Gu Cuicui , Gao Sijia , Li Man , Zhang Haixiang , Zhu Shidong 

TITLE=Complete mitochondrial genome assembly of Zizania latifolia and comparative genome analysis

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1381089

DOI=10.3389/fpls.2024.1381089

ISSN=1664-462X

ABSTRACT=<p><italic>Zizania latifolia</italic> (Griseb.) Turcz. ex Stapf has been cultivated as a popular aquatic vegetable in China due to its important nutritional, medicinal, ecological, and economic values. The complete mitochondrial genome (mitogenome) of <italic>Z. latifolia</italic> has not been previously studied and reported, which has hindered its molecular systematics and understanding of evolutionary processes. Here, we assembled the complete mitogenome of <italic>Z. latifolia</italic> and performed a comprehensive analysis including genome organization, repetitive sequences, RNA editing event, intercellular gene transfer, phylogenetic analysis, and comparative mitogenome analysis. The mitogenome of <italic>Z. latifolia</italic> was estimated to have a circular molecule of 392,219 bp and 58 genes consisting of three rRNA genes, 20 tRNA genes, and 35 protein-coding genes (PCGs). There were 46 and 20 simple sequence repeats (SSRs) with different motifs identified from the mitogenome and chloroplast genome of <italic>Z. latifolia</italic>, respectively. Furthermore, 49 homologous fragments were observed to transfer from the chloroplast genome to the mitogenome of <italic>Z. latifolia</italic>, accounting for 47,500 bp, presenting 12.1% of the whole mitogenome. In addition, there were 11 gene-containing homologous regions between the mitogenome and chloroplast genome of <italic>Z. latifolia</italic>. Also, approximately 85% of fragments from the mitogenome were duplicated in the <italic>Z. latifolia</italic> nuclear genome. Selection pressure analysis revealed that most of the mitochondrial genes were highly conserved except for <italic>ccmFc</italic>, <italic>ccmFn</italic>, <italic>matR</italic>, <italic>rps1</italic>, and <italic>rps3</italic>. A total of 93 RNA editing sites were found in the PCGs of the mitogenome. <italic>Z. latifolia</italic> and <italic>Oryza minuta</italic> are the most closely related, as shown by collinear analysis and the phylogenetic analysis. We found that repeat sequences and foreign sequences in the mitogenomes of Oryzoideae plants were associated with genome rearrangements. In general, the availability of the <italic>Z. latifolia</italic> mitogenome will contribute valuable information to our understanding of the molecular and genomic aspects of <italic>Zizania</italic>.</p>