AUTHOR=Yan Kuan , Zhu Hui , Cao Guiling , Meng Lina , Li Junqiang , Zhang Jian , Liu Sicen , Wang Yujie , Feng Ruizhang , Soaud Salma A. , Elhamid Mohamed A. Abd , Heakel Rania M. Y. , Wei Qin , El-Sappah Ahmed H. , Ru Dafu TITLE=Chromosome genome assembly of the Camphora longepaniculata (Gamble) with PacBio and Hi-C sequencing data JOURNAL=Frontiers in Plant Science VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1372127 DOI=10.3389/fpls.2024.1372127 ISSN=1664-462X ABSTRACT=Introduction

Camphora longepaniculata, a crucial commercial crop and a fundamental component of traditional Chinese medicine, is renowned for its abundant production of volatile terpenoids. However, the lack of available genomic information has hindered pertinent research efforts in the past.

Methods

To bridge this gap, the present study aimed to use PacBio HiFi, short-read, and highthroughput chromosome conformation capture sequencing to construct a chromosome-level assembly of the C. longepaniculata genome.

Results and discussion

With twelve chromosomes accounting for 99.82% (766.69 Mb) of the final genome assembly, which covered 768.10 Mb, it was very complete. Remarkably, the assembly’s contig and scaffold N50 values are exceptional as well—41.12 and 63.78 Mb, respectively—highlighting its excellent quality and intact structure. Furthermore, a total of 39,173 protein-coding genes were predicted, with 38,766 (98.96%) of them being functionally annotated. The completeness of the genome was confirmed by the Benchmarking Universal Single-Copy Ortholog evaluation, which revealed 99.01% of highly conserved plant genes. As the first comprehensive assembly of the C. longepaniculata genome, it provides a crucial starting point for deciphering the complex pathways involved in terpenoid production. Furthermore, this excellent genome serves as a vital resource for upcoming research on the breeding and genetics of C. longepaniculata.