AUTHOR=Gai Weiling , Liu Chunying , Yang Mengjie , Li Feng , Xin Hua , Gai Shupeng 

TITLE=Calcium signaling facilitates chilling- and GA- induced dormancy release in tree peony

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1362804

DOI=10.3389/fpls.2024.1362804

ISSN=1664-462X

ABSTRACT=<p>Calcium plays a crucial role in plant growth and development, yet little is known about its function in endodormancy regulation. Tree peony (<italic>Paeonia suffruticosa</italic>), characterized by compound buds and large flowers, is well-known for its ornamental and medicinal value. To break bud dormancy release is a prerequisite of flowering and forcing culture, particularly during the Spring Festival. In this study, the Ca<sup>2+</sup> chelator EGTA and Ca<sup>2+</sup> channel blocker LaCl<sub>3</sub> were applied, resulting in a significant delay in budburst during both chilling- and gibberellin (GA)- induced dormancy release in a dosage-dependent manner. As expected, the retardation of bud break was recovered by the supplementation of 30 mM CaCl<sub>2</sub>, indicating a facilitating role of calcium in dormancy release. Accordingly, several calcium-sensor-encoding genes including <italic>Calmodulin</italic> (<italic>CaM</italic>) and <italic>Ca<sup>2+</sup>-dependent protein kinases</italic> (<italic>CDPK</italic>s) were significantly up-regulated by prolonged chilling and exogenous GAs. Ultrastructure observations revealed a decline in starch grains and the reopening of transport corridors following prolonged chilling. Calcium deposits were abundant in the cell walls and intercellular spaces at the early dormant stage but were enriched in the cytosol and nucleus before dormancy release. Additionally, several genes associated with dormancy release, including <italic>EBB1</italic>, <italic>EBB3</italic>, <italic>SVP</italic>, <italic>GA20ox</italic>, <italic>RGL1</italic>, <italic>BG6</italic>, and <italic>BG9</italic>, were differentially expressed after calcium blocking and recovery treatments, indicating that calcium might partially modulate dormancy release through GA and ABA pathways. Our findings provide novel insights into the mechanism of dormancy release and offer potential benefits for improving and perfecting forcing culture technology in tree peonies.</p>