AUTHOR=Singh Davinder , Ziems Laura , Chettri Mumta , Dracatos Peter , Forrest Kerrie , Bhavani Sridhar , Singh Ravi , Barnes Charles W. , Zapata Patricio Javier Noroña , Gangwar Om , Kumar Subodh , Bhardwaj Subhash , Park Robert F. 

TITLE=Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1352402

DOI=10.3389/fpls.2024.1352402

ISSN=1664-462X

ABSTRACT=<p>Barley stripe or yellow rust (BYR) caused by <italic>Puccinia striiformis</italic> f. sp. <italic>hordei</italic> (<italic>Psh</italic>) is a significant constraint to barley production. The disease is best controlled by genetic resistance, which is considered the most economical and sustainable component of integrated disease management. In this study, we assessed the diversity of resistance to <italic>Psh</italic> in a panel of international barley genotypes (n = 266) under multiple disease environments (Ecuador, India, and Mexico) using genome-wide association studies (GWASs). Four quantitative trait loci (QTLs) (three on chromosome 1H and one on 7H) associated with resistance to <italic>Psh</italic> were identified. The QTLs were validated by mapping resistance to <italic>Psh</italic> in five biparental populations, which detected key genomic regions on chromosomes 1H (populations Pompadour/Zhoungdamei, Pompadour/Zug161, and CI9214/Baudin), 3H (Ricardo/Gus), and 7H (Fumai8/Baronesse). The QTL <italic>RpshQ.GWA.1H.1</italic> detected by GWAS and <italic>RpshQ.Bau.1H</italic> detected using biparental mapping populations co-located were the most consistent and stable across environments and are likely the same resistance region. <italic>RpshQ.Bau.1H</italic> was saturated using population CI9214/Baudin by enriching the target region, which placed the resistance locus between 7.9 and 8.1 Mbp (flanked by markers <italic>sun_B1H_03</italic>, 0.7 cM proximal to <italic>Rpsh_1H</italic> and <italic>sun_B1H_KASP_02</italic>, 3.2 cM distal on 1HS) in the Morex reference genome v.2. A Kompetitive Allele Specific PCR (KASP) marker <italic>sun_B1H_KASP_01</italic> that co-segregated for <italic>RpshQ.Bau.1H</italic> was developed. The marker was validated on 50 Australian barley cultivars, showing well-defined allelic discrimination and presence in six genotypes (Baudin, Fathom, Flagship, Grout, Sakurastar, and Shepherd). This marker can be used for reliable marker-assisted selection and pyramiding of resistance to <italic>Psh</italic> and in diversifying the genetic base of resistance to stripe rust.</p>