Skip to main content

ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1352402

Genetic mapping of stripe rust resistance in a geographically diverse barley collection and in selected biparental populations

Provisionally accepted
Davinder Singh Davinder Singh 1*Laura A. Ziems Laura A. Ziems 1Mumta Chettri Mumta Chettri 1Peter Dracatos Peter Dracatos 2Kerrie Forrest Kerrie Forrest 3Sridhar Bhavani Sridhar Bhavani 4Ravi P. Singh Ravi P. Singh 5Charles W. Barnes Charles W. Barnes 6Patricio J. Noroña Zapata Patricio J. Noroña Zapata 7Om P. Gangwar Om P. Gangwar 8Subodh Kumar Subodh Kumar 8Subhash C. Bhardwaj Subhash C. Bhardwaj 8Robert F. Park Robert F. Park 1,9
  • 1 Plant Breeding Institute, Faculty of Science, The University of Sydney, Sydney, New South Wales, Australia
  • 2 La Trobe University, Melbourne, Victoria, Australia
  • 3 Agriculture Victoria AgriBio, Department of Energy, Environment and Climate Action (DEECA), Bundoora, Victoria, Australia
  • 4 The International Maize and Wheat Improvement Center (CIMMYT), Nairobi, Kenya
  • 5 International Maize and Wheat Improvement Center (Mexico), Texcoco, Tabasco, Mexico
  • 6 USDA Forest Service, Forest Health Protection, San Bernardino, CA, 92408, United States
  • 7 Instituto Nacional de Investigaciones Agropecuarias (INIAP), Quito, Ecuador
  • 8 Indian Institute of Wheat and Barley Research, Regional Station Flowerdale, Shimla, India
  • 9 The University of Sydney, Darlington, New South Wales, Australia

The final, formatted version of the article will be published soon.

    Barley stripe or yellow rust (BYR) caused by Puccinia striiformis f. sp. hordei (Psh) is a significant constraint to barley production. The disease is best controlled by genetic resistance, which is considered the most economical and sustainable component of integrated disease management. In this study, we assessed diversity of resistance to Psh in a panel of international barley genotypes (n = 266) under multiple disease environments (Ecuador, India, and Mexico) using genome wide association studies (GWAS). Four QTL (three on chromosome 1H and one on 7H) associated with resistance to Psh were identified. The QTL were validated by mapping resistance to Psh in five biparental populations, which detected key genomic regions on chromosomes 1H (populations Pompadour/Zhoungdamei, Pomapadour/Zug161, CI9214/Baudin), 3H (Ricardo/Gus) and 7H (Fumai8/Baronesse). QTL RpshQ.GWA.1H.1 detected by GWAS and RpshQ.Bau.1H detected using biparental mapping populations colocated, were the most consistent and stable across environments and are likely the same resistance region. RpshQ.Bau.1H was saturated using population CI9214/Baudin by enriching the target region, which placed the resistance locus between 7.9 and 8.1 Mbp (flanked by markers sun_B1H_03, 0.7 cM proximal to Rpsh_1H and sun_B1H_KASP_02, 3.2cM distal on 1HS) in the Morex reference genome v.2. A KASP marker sun_B1H_KASP_01 that cosegregated for RpshQ.Bau.1H was developed. The marker was validated on 50 Australian barley cultivars, showing well-defined allelic discrimination and presence in six genotypes (Baudin, Fathom, Flagship, Grout, Sakurastar and Shepherd). This marker can be used for reliable marker assisted selection and pyramiding of resistance to Psh, and in diversifying the genetic base of resistance to stripe rust.

    Keywords: barley, Yellow rust, Puccinia striiformis f. sp. hordei, GWAS, Mapping, QTL, marker assisted selection Formatted: Line spacing: single Deleted: Seven Deleted:, Deleted: single QTL on 2H

    Received: 08 Dec 2023; Accepted: 28 Jun 2024.

    Copyright: © 2024 Singh, Ziems, Chettri, Dracatos, Forrest, Bhavani, Singh, Barnes, Noroña Zapata, Gangwar, Kumar, Bhardwaj and Park. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Davinder Singh, Plant Breeding Institute, Faculty of Science, The University of Sydney, Sydney, 2570, New South Wales, Australia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.