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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1344155
Transcriptome and metabolome analyses provide crucial insights into the adaptation of chieh-qua to Fusarium oxysporum infection
Provisionally accepted
Yanchun Qiao
1*
Jiazhu Peng
1*
Ming Wang
2*
Guoping He
1*
Qingwu Peng
2*
Bei Wu
1
Yuping Liu
1*
Songguang Yang
2
Xiuchun Dai
1*- 1 Guangzhou Academy of Agricultural Sciences, Guangzhou, China
- 2 Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, Guangdong Province, China
Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) is a wax gourd variety that is generally susceptible to infection and damage by Fusarium oxysporum during its cultivation. Therefore, analyzing the adaption mechanism of chieh-qua to F.Oxysporum infection is of great significance for cultivating resistant varieties.Through comparative transcriptome analysis between F. Oxysporum infected samples and control samples of susceptible lines, this study found that proteins such as NPR1, TGA and PR1 in plant hormone signal transduction pathway were up-regulated after infection, which may activate a series of plant secondary metabolic synthesis pathways. In addition, the expression of 27 genes in the flavonoid biosynthetic process in resistant lines after infection was significantly higher than that in susceptible lines, indicating that these genes may be involved in fungal resistance.This study also found that alternative splicing of genes may play an important role in responding to F. Oxysporum infection. For example, plant protein kinase genes such as EDR1, SRK2E and KIPK1 were not differentially expressed after F. Oxysporum infection, but the transcripts they produced differ at the transcription level. Finally, through comparative metabolome analysis, this study identified potentially functional substances such as oxalic acid that increased in content after F. Oxysporum infection.Through integrated analysis of transcriptome and metabolome, some differential expressed genes significantly related to differential metabolites were also identified.This study provides a basis for understanding and utilizing chieh-qua's infection mechanism of F. Oxysporum through analysis of the transcriptome and metabolome.
Keywords: Chieh-qua, Fusarium oxysporum, Differentially expressed, Metabolome, Alternative Splicing
Received: 25 Nov 2023; Accepted: 11 Sep 2024.
Copyright: © 2024 Qiao, Peng, Wang, He, Peng, Wu, Liu, Yang and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yanchun Qiao, Guangzhou Academy of Agricultural Sciences, Guangzhou, China
Jiazhu Peng, Guangzhou Academy of Agricultural Sciences, Guangzhou, China
Ming Wang, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, 510640, Guangdong Province, China
Guoping He, Guangzhou Academy of Agricultural Sciences, Guangzhou, China
Qingwu Peng, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, 510640, Guangdong Province, China
Yuping Liu, Guangzhou Academy of Agricultural Sciences, Guangzhou, China
Xiuchun Dai, Guangzhou Academy of Agricultural Sciences, Guangzhou, China
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