AUTHOR=Han Bei , Yan Junjun , Wu Tao , Yang Xinyu , Wang Yajie , Ding Guangda , Hammond John , Wang Chuang , Xu Fangsen , Wang Sheliang , Shi Lei 

TITLE=Proteomics reveals the significance of vacuole Pi transporter in the adaptability of Brassica napus to Pi deprivation

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1340867

DOI=10.3389/fpls.2024.1340867

ISSN=1664-462X

ABSTRACT=<p>Vacuolar Pi transporters (VPTs) have recently been identified as important regulators of cellular Pi status in <italic>Arabidopsis thaliana</italic> and <italic>Oryza sativa</italic>. In the oil crop <italic>Brassica napus</italic>, <italic>BnA09PHT5;1a</italic> and <italic>BnC09PHT5;1a</italic> are two homologs of <italic>AtPHT5;1</italic>, the vacuolar Pi influx transporter in <italic>Arabidopsis</italic>. Here, we show that Pi deficiency induces the transcription of both homologs of <italic>PHT5;1a</italic> genes in <italic>B. napus</italic> leaves. <italic>Brassica PHT5;1a</italic> double mutants (DM) had smaller shoots and higher cellular Pi concentrations than wild-type (WT, Westar 10), suggesting the potential role of <italic>BnPHT5;1a</italic> in modulating cellular Pi status in <italic>B. napus</italic>. A proteomic analysis was performed to estimate the role of <italic>BnPHT5;1a</italic> in Pi fluctuation. Results show that Pi deprivation disturbs the abundance of proteins in the physiological processes involved in carbohydrate metabolism, response to stimulus and stress in <italic>B. napus</italic>, while disruption of <italic>BnPHT5;1a</italic> genes may exacerbate these processes. Besides, the processes of cell redox homeostasis, lipid metabolic and proton transmembrane transport are supposed to be unbalanced in <italic>BnPHT5;1a</italic> DM under the -Pi condition. Noteworthy, disruption of <italic>BnPHT5;1a</italic> genes severely alters the abundance of proteins related to ATP biosynthesis, and proton/inorganic cation transmembrane under normal Pi condition, which might contribute to <italic>B. napus</italic> growth limitations. Additionally, seven new protein markers of Pi homeostasis are identified in <italic>B. napus</italic>. Taken together, this study characterizes the important regulatory role of <italic>BnPHT5;1a</italic> genes as vacuolar Pi influx transporters in Pi homeostasis in <italic>B. napus</italic>.</p>