AUTHOR=Rajendran Ambika , Ramlal Ayyagari , Sarkar Subham , Agasti Sarit S. , Rajarajan K. , Lal S. K. , Raju Dhandapani , Subramaniam Sreeramanan 

TITLE=Autofluorescence−spectral imaging for rapid and invasive characterization of soybean for pre-germination anaerobic stress tolerance

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1334909

DOI=10.3389/fpls.2024.1334909

ISSN=1664-462X

ABSTRACT=<p>The autofluorescence-spectral imaging (ASI) technique is based on the light-emitting ability of natural fluorophores. Soybean genotypes showing contrasting tolerance to pre-germination anaerobic stress can be characterized using the photon absorption and fluorescence emission of natural fluorophores occurring in seed coats. In this study, tolerant seeds were efficiently distinguished from susceptible genotypes at 405 nm and 638 nm excitation wavelengths. ASI approach can be employed as a new marker for the detection of photon-emitting compounds in the tolerant and susceptible soybean seed coats. Furthermore, the accuracy of rapid characterization of genotypes using this technique can provide novel insights into soybean breeding.</p>