AUTHOR=Yan Yadan , Wen Yafeng , Wang Ye , Wu Xingtong , Li Xinyu , Wang Chuncheng , Zhao Yanghui 

TITLE=Metabolome integrated with transcriptome reveals the mechanism of three different color formations in Taxus mairei arils

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1330075

DOI=10.3389/fpls.2024.1330075

ISSN=1664-462X

ABSTRACT=<p>Maire yew (<italic>Taxus mairei</italic>), an evergreen conifer, has high ornamental and medicinal value. The arils of this species has three different colors. However, the variation mechanisms of arils color formation remains unclear. Here, the gene expression and metabolite concentration were profiled for red (RTM), yellow (YTM), and purple (PTM) arils in different developmental stages. A total of 266 flavonoids and 35 carotenoids were identified. The predominant pigments identified in YTM were epiafzelechin, lutein, and β-Cryptoxanthin, while malvidin-3,5-di-O-glucoside and apigenin played crucial roles in PTM. And significant differential expression was observed among the <italic>HCT</italic>, <italic>DFR</italic>, <italic>LAR</italic>, <italic>ANS</italic>, <italic>crtB</italic>, <italic>NCED</italic>, and <italic>CCoAOMT</italic> genes across different color arils. During the maturation of yellow arils, the upregulation of <italic>HCT</italic> was strongly correlated with the accumulation of epiafzelechin. The diminished expression of <italic>DFR</italic>, <italic>LAR</italic>, and <italic>ANS</italic> seemed to inhibit the production of delphinidin-3-O-rutinoside. The decrease in <italic>crtB</italic> expression and concurrent increase in <italic>NCED</italic> expression potentially regulate the heightened accumulation of lutein. Meanwhile, the accumulation of β-cryptoxanthin appeared seemed to be positively influenced by <italic>NCED.</italic> As aril turning purple, the decreased expression of <italic>CCoAOMT</italic> seemed to facilitate the synthesis of apigenin. The substantial upregulation of <italic>DFR</italic> promoted the production of malvidin-3,5-di-O-glucoside. Additionally, the overexpression of MYBs may plays the important role in regulating the formation of different colored arils. In total, 14 genes were selected for qRT-PCR validation, the results indicated the reliability of the transcriptome sequences data. Our findings could provide valuable insight into the molecular breeding, development, and application of Maire yew resources.</p>