AUTHOR=Zhong Shumin , Guo Chuibao , Su Lu , Jiang Han , Wang Xue-er , Shi Li , Li Xiaogang , Liao Xiaolan , Xue Jin 

TITLE=Physiological and transcriptomic analyses provide preliminary insights into the autotoxicity of Lilium brownii

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1330061

DOI=10.3389/fpls.2024.1330061

ISSN=1664-462X

ABSTRACT=<p><italic>Lilium brownii</italic> F. E. Brown ex Miellez var. viridulum Baker (Longya lily) is a variety of <italic>Lilium brownii</italic> F.E. Br. ex Miellez. We used HS-SPME and GC-MS to screened the tissues of <italic>L. brownii</italic> roots, stems, bulbs, and leaves and obtained 2,4-DTBP as an autotoxic substance for subsequent analysis. 2,4-DTBP was highly autotoxic in some treatment groups. Based on changes in physiological indicators, we carried out transcriptomic analysis to investigate the mechanisms of autotoxicity of substances on <italic>L. brownii</italic> and obtained 188,505 Unigenes. GO and KEGG enrichment analyses showed that <italic>L. brownii</italic> responded differently to different concentrations and treatment times of 2,4-DTBP. We observed significant changes in genes associated with ROS, phytohormones, and MAPK signaling cascades. 2,4-DTBP affects chloroplasts, the integrity of the respiratory electron transport chain, and ribosomes, causing <italic>L. brownii</italic> autotoxicity. Our findings provide a practical genomic resource for future research on <italic>L. brownii</italic> autotoxicity and evidence for the mechanism of action of autotoxic substances.</p>