AUTHOR=Yang Xiao-Long , Ma Xiao-Fei , Ye Zi-Piao , Yang Long-Sheng , Shi Jun-Bo , Wang Xun , Zhou Bei-Bei , Wang Fu-Biao , Deng Zi-Fa 

TITLE=Simulating short-term light responses of photosynthesis and water use efficiency in sweet sorghum under varying temperature and CO2 conditions

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1291630

DOI=10.3389/fpls.2024.1291630

ISSN=1664-462X

ABSTRACT=<p>Climate change, characterized by rising atmospheric CO<sub>2</sub> levels and temperatures, poses significant challenges to global crop production. Sweet sorghum, a prominent C<sub>4</sub> cereal extensively grown in arid areas, emerges as a promising candidate for sustainable bioenergy production. This study investigated the responses of photosynthesis and leaf-scale water use efficiency (WUE) to varying light intensity (<italic>I</italic>) in sweet sorghum under different temperature and CO<sub>2</sub> conditions. Comparative analyses were conducted between the <italic>A</italic><sub>n</sub>-<italic>I</italic>, <italic>g</italic><sub>s</sub>-<italic>I</italic>, <italic>T</italic><sub>r</sub>-<italic>I</italic>, WUE<sub>i</sub>-<italic>I</italic>, and WUE<sub>inst</sub>-<italic>I</italic> models proposed by Ye et al. and the widely utilized the non-rectangular hyperbolic (NRH) model for fitting light response curves. The Ye's models effectively replicated the light response curves of sweet sorghum, accurately capturing the diminishing intrinsic WUE (WUE<sub>i</sub>) and instantaneous WUE (WUE<sub>inst</sub>) trends with increasing <italic>I</italic>. The fitted maximum values of <italic>A</italic><sub>n</sub>, <italic>g</italic><sub>s</sub>, <italic>T</italic><sub>r</sub>, WUE<sub>i</sub>, and WUE<sub>inst</sub> and their saturation light intensities closely matched observations, unlike the NRH model. Despite the NRH model demonstrating high <italic>R</italic><sup>2</sup> values for <italic>A</italic><sub>n</sub>-<italic>I</italic>, <italic>g</italic><sub>s</sub>-<italic>I</italic>, and <italic>T</italic><sub>r</sub>-<italic>I</italic> modelling, it returned the maximum values significantly deviating from observed values and failed to generate saturation light intensities. It also inadequately represented WUE responses to <italic>I</italic>, overestimating WUE. Across different leaf temperatures, <italic>A</italic><sub>n</sub>, <italic>g</italic><sub>s</sub>, and <italic>T</italic><sub>r</sub> of sweet sorghum displayed comparable light response patterns. Elevated temperatures increased maximum <italic>A</italic><sub>n</sub>, <italic>g</italic><sub>s</sub>, and <italic>T</italic><sub>r</sub> but consistently declined maximum WUE<sub>i</sub> and WUE<sub>inst</sub>. However, WUE<sub>inst</sub> declined more sharply due to the disproportionate transpiration increase over carbon assimilation. Critically, sweet sorghum <italic>A</italic><sub>n</sub> saturated at current atmospheric CO<sub>2</sub> levels, with no significant gains under 550 μmol mol<sup>−1</sup>. Instead, stomatal closure enhanced WUE under elevated CO<sub>2</sub> by coordinated <italic>g</italic><sub>s</sub> and <italic>T</italic><sub>r</sub> reductions rather than improved carbon assimilation. Nonetheless, this response diminished under simultaneously high temperature, suggesting intricate interplay between CO<sub>2</sub> and temperature in modulating plant responses. These findings provide valuable insights into photosynthetic dynamics of sweet sorghum, aiding predictions of yield and optimization of cultivation practices. Moreover, our methodology serves as a valuable reference for evaluating leaf photosynthesis and WUE dynamics in diverse plant species.</p>