AUTHOR=Pingarron-Cardenas Gabriela , Onkokesung Nawaporn , Goldberg-Cavalleri Alina , Lange Gudrun , Dittgen Jan , Edwards Robert 

TITLE=Selective herbicide safening in dicot plants: a case study in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1335764

DOI=10.3389/fpls.2023.1335764

ISSN=1664-462X

ABSTRACT=<p>Safeners are agrochemicals co-applied with herbicides that facilitate selective control of weeds by protecting monocot crops from chemical injury through enhancing the expression of detoxifying enzymes such as glutathione transferases (GSTs). Even though the application of safeners causes the induction of genes encoding GSTs in model dicots such as <italic>Arabidopsis thaliana</italic>, safeners do not protect broadleaf crops from herbicide injury. In this study, we proposed that the localized induction of <italic>Arabidopsis</italic> GSTs and the fundamental differences in their detoxifying activity between dicot and monocot species, underpin the failure of safeners to protect <italic>Arabidopsis</italic> from herbicide toxicity. Using the herbicide safener, isoxadifen-ethyl, we showed that three tau (U) family GSTs namely <italic>AtGSTU7</italic>, <italic>AtGSTU19</italic> and <italic>AtGSTU24</italic> were induced with different magnitude by isoxadifen treatment in root and rosette tissues. The higher magnitude of inducibility of these <italic>AtGSTUs</italic> in the root tissues coincided with the enhanced metabolism of flufenacet, a herbicide that is active in root tissue, protecting <italic>Arabidopsis</italic> plants from chemical injury. Assay of the recombinant enzyme activities and the significant reduction in flufenacet metabolism determined in the T-DNA insertion mutant of <italic>AtGSTU7</italic> (<italic>gstu7</italic>) in <italic>Arabidopsis</italic> plants identified an important function for <italic>At</italic>GSTU7 protein in flufenacet detoxification. <italic>In-silico</italic> structural modeling of <italic>At</italic>GSTU<italic>7</italic>, suggested the unique high activity of this enzyme toward flufenacet was due to a less constrained active site compared to <italic>At</italic>GSTU19 and <italic>At</italic>GSTU24. We demonstrate here that it is possible to induce herbicide detoxification in dicotyledonous plants by safener treatment, albeit with this activity being restricted to very specific combinations of herbicide chemistry, and the localized induction of enzymes with specific detoxifying activities.</p>