AUTHOR=Wang Chubiao , Luo Jianzhong , He Wenliang , Huang Anying , Lu Wanhong , Lin Yan , Ou Yuduan 

TITLE=Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN in Corymbia citriodora

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1308354

DOI=10.3389/fpls.2023.1308354

ISSN=1664-462X

ABSTRACT=<p>The GDP-D-mannose pyrophosphorylase (<italic>GMP</italic>) and microtubule severing enzyme KATANIN (<italic>KTN</italic>) are crucial for wood formation. Although functional identification has been performed in <italic>Arabidopsis</italic>, few comprehensive studies have been conducted in forest trees. In this study, we discovered 8 <italic>CcGMP</italic> and 4 <italic>CcKTN</italic> genes by analyzing the whole genome sequence of <italic>Corymbia citriodora</italic>. The chromosomal location, genome synteny, phylogenetic relationship, protein domain, motif identification, gene structure, cis-acting regulatory elements, and protein-interaction of <italic>CcGMP</italic> and <italic>CcKTN</italic> were all investigated. <italic>KTN</italic> has just one pair of segmentally duplicated genes, while <italic>GMP</italic> has no duplication events. According to gene structure, two 5’ UTRs were identified in <italic>CcGMP4</italic>. Furthermore, there is no protein-interaction between KTN and GMP. Based on real-time PCR, the expression of most genes showed a positive connection with DBH diameters. In addition, the expression of <italic>CcGMP4</italic> and <italic>CcKTN4</italic> genes were greater in different size tree, indicating that these genes are important in secondary xylem production. Overall, this findings will enhance our comprehension of the intricacy of <italic>CcGMP</italic>&amp;<italic>CcKTN</italic> across diverse DBHs and furnish valuable insights for future functional characterization of specific genes in <italic>C. citriodora.</italic></p>