AUTHOR=Liu Chenqi , Li Liang , Yang Shuo , Wang Mingye , Zhang Hang , Li Si 

TITLE=Multi-omic insights into the cellular response of Phaeodactylum tricornutum (Bacillariophyta) strains under grazing pressure

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1308085

DOI=10.3389/fpls.2023.1308085

ISSN=1664-462X

ABSTRACT=<sec><title>Background/Aims</title><p><italic>Phaeodactylum tricornutum</italic>, a model organism of diatoms, plays a crucial role in Earth’s primary productivity. Investigating its cellular response to grazing pressure is highly significant for the marine ecological environment. Furthermore, the integration of multi-omics approaches has enhanced the understanding of its response mechanism.</p></sec><sec><title>Methods</title><p>To assess the molecular and cellular responses of <italic>P.tricornutum</italic> to grazer presence, we conducted transcriptomic, proteomic, and metabolomic analyses, combined with phenotypic data from previous studies. Sequencing data were obtained by Illumina RNA sequencing, TMT Labeled Quantitative Proteomics and Non-targeted Metabolomics, and WGCNA analysis and statistical analysis were performed.</p></sec><sec><title>Results</title><p>Among the differentially expressed genes, we observed complex expression patterns of the core genes involved in the phenotypic changes of <italic>P.tricornutum</italic> under grazing pressure across different strains and multi-omics datasets. These core genes primarily regulate the levels of various proteins and fatty acids, as well as the cellular response to diverse signals.</p></sec><sec><title>Conclusion</title><p>Our research reveals the association of multi-omics in four strains responses to grazing effects in <italic>P.tricornutum</italic>. Grazing pressure significantly impacted cell growth, fatty acid composition, stress response, and the core genes involved in phenotype transformation.</p></sec>