AUTHOR=Jin Yirong , Wang Yamei , Liu Jindong , Wang Fuyan , Qiu Xiaodong , Liu Peng 

TITLE=Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.)

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1274392

DOI=10.3389/fpls.2023.1274392

ISSN=1664-462X

ABSTRACT=<p>Identifying loci for root system architecture (RSA) traits and developing available markers are crucial for wheat breeding. In this study, RSA-related traits, including total root length (TRL), total root area (TRA), and number of root tips (NRT), were evaluated in the Doumai/Shi4185 recombinant inbred line (RIL) population under hydroponics. In addition, both the RILs and parents were genotyped using the wheat 90K single-nucleotide polymorphism (SNP) array. In total, two quantitative trait loci (QTLs) each for TRL (<italic>QTRL.caas-4A.1</italic> and <italic>QTRL.caas-4A.2</italic>), TRA (<italic>QTRA.caas-4A</italic> and <italic>QTRA.caas-4D</italic>), and NRT (<italic>QNRT.caas-5B</italic> and <italic>QNRT.caas-5D</italic>) were identified and each explaining 5.94%–9.47%, 6.85%–7.10%, and 5.91%–10.16% phenotypic variances, respectively. Among these, <italic>QTRL.caas-4A.1</italic> and <italic>QTRA.caas-4A</italic> overlapped with previous reports, while <italic>QTRL.caas-4A.2</italic>, <italic>QTRA.caas-4D</italic>, <italic>QNRT.caas-5B</italic>, and <italic>QNRT.caas-5D</italic> were novel. The favorable alleles of <italic>QTRL.caas-4A.1</italic>, <italic>QTRA.caas-4A</italic>, and <italic>QTRA.caas-5B</italic> were contributed by Doumai, whereas the favorable alleles of <italic>QTRL.caas-4A.2</italic>, <italic>QTRA.caas-4D</italic>, and <italic>QTRA.caas-5D</italic> originated from Shi 4185. Additionally, two competitive allele-specific PCR (KASP) markers, <italic>Kasp_4A_RL</italic> (<italic>QTRA.caas-4A</italic>) and <italic>Kasp_5D_RT</italic> (<italic>QNRT.caas-5D</italic>), were developed and validated in 165 wheat accessions. This study provides new loci and available KASP markers, accelerating wheat breeding for higher yields.</p>