AUTHOR=Pinski Artur , Betekhtin Alexander 

TITLE=Efficient Agrobacterium-mediated transformation and genome editing of Fagopyrum tataricum

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1270150

DOI=10.3389/fpls.2023.1270150

ISSN=1664-462X

ABSTRACT=<p><italic>Fagopyrum tataricum</italic> (L.) Gaertn. is an exceptional crop known for its remarkable health benefits, high levels of beneficial polyphenols and gluten-free properties, making it highly sought-after as a functional food. Its self-fertilisation capability and adaptability to challenging environments further contribute to its potential as a sustainable agricultural option. To harness its unique traits, genetic transformation in <italic>F. tataricum</italic> is crucial. In this study, we optimised the Agrobacterium-mediated transformation protocol for <italic>F. tataricum</italic> callus, resulting in a transformation rate of regenerated plants of approximately 20%. The protocol’s effectiveness was confirmed through successful GUS staining, GFP expression, and the generation of albino plants via <italic>FtPDS</italic> gene inactivation. These results validate the feasibility of genetic manipulation and highlight the potential for trait enhancement in <italic>F. tataricum</italic>.</p>