AUTHOR=Li Ling , Liang Yuchen , Liu Yinan , Sun Zeyi , Liu Yuning , Yuan Zening , Fu Chang 

TITLE=Transcriptome analyses reveal photosynthesis-related genes involved in photosynthetic regulation under low temperature stress in Lavandula angustifolia Mill.

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1268666

DOI=10.3389/fpls.2023.1268666

ISSN=1664-462X

ABSTRACT=<p>In order to reveal the mechanisms of photosynthetic regulation of <italic>Lavandula angustifolia</italic> Mill. under low temperature stress, photosynthesis-related genes were screened and the molecular mechanism were analyzed for this species growing in Harbin, northeast of China. RNA-seq technique and photosynthetic physiology measurement were performed under 20°C, 10°C, and 0°C in this study. The results showed that the observing modified rectangular hyperbola mode could accurately reflect the light-response processes under low temperature stress and the low temperature reduced the light energy utilization of <italic>L. angustifolia</italic>. The stomatal conductance decreased with the temperature dropping, which was associated with the up-regulation of <italic>LaBAM1s</italic>, <italic>LaMPK4-1</italic> and <italic>LaMMK2</italic>. The up-regulation of <italic>LaMPK4-1</italic> and <italic>LaMMK2</italic> was beneficial for ROS scavenging. The improvement of cold resistance in <italic>L. angustifolia</italic> was related to the up-regulated expression of <italic>LaFBA</italic> and <italic>LaOMTs</italic> and down-regulated expression of <italic>LaGAPAs</italic>, <italic>LaGOX</italic>, and <italic>LaTKL1s</italic> with the temperature decreasing. The up-expression of <italic>LaPSY</italic> at 10°C than it at 20°C could protect the photosynthetic organs from oxidative damage. Moreover, the photosynthetic rates at 10°C and 0°C were close to the measured values, which was related to the interactions of RCA with SBPase and Rubisco with SBPase. These findings could provide a theoretical reference for further exploring the cold tolerance mechanism of <italic>L. angustifolia</italic>, as an important aromatic plant resource, and promoting its cultivation and distribution in the northeast of China.</p>