AUTHOR=Navarre Catherine , Orval Rik , Peeters Marie , Bailly Nicolas , Chaumont François 

TITLE=Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1266775

DOI=10.3389/fpls.2023.1266775

ISSN=1664-462X

ABSTRACT=<p>Several recombinant proteins have been successfully produced in plants. This usually requires <italic>Agrobacterium</italic>-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via <italic>A. tumefaciens</italic>, especially when the protein of interest is toxic to the bacteria. In particular, we found that a Tn3 transposon can be transferred from the pAL4404 Ti plasmid of <italic>A. tumefaciens</italic> LBA4404 into the expression cassette when using the widely adopted <italic>35S</italic> promoter, thereby damaging T-DNA and preventing correct expression of the gene of interest in <italic>Nicotiana tabacum</italic> BY-2 suspension cells.</p>