AUTHOR=Li Xinchun , Hou Huihui , Li Bin , Guo Shiping , Jiang Lianqiang , Xu Chuantao , Xie Yunbo , An Mengnan , Zhang Chong , Wu Yuanhua 

TITLE=Identification and physiological activity of (methoxymethyl)triphenylphosphonium chloride as a new phytotoxin isolated from Rhizoctonia solani AG-3 TB

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1264567

DOI=10.3389/fpls.2023.1264567

ISSN=1664-462X

ABSTRACT=<p><italic>Rhizoctonia solani</italic> as a cosmopolitan fungus is the causative agent of many crop diseases and leads to significant economic losses in crop production. To explore the toxin structure and physiological activity of <italic>R. solani</italic> AG-3 TB, high-performance liquid chromatography (HPLC), infrared absorption spectrum (IR), and nuclear magnetic resonance spectrum (NMR) were required. Here, the compound (methoxymethyl)triphenylphosphonium chloride (MMC) with the molecular formula C<sub>20</sub>H<sub>20</sub>ClOP was purified and identified from <italic>R. solani</italic> AG-3 TB. The pure compound MMC treated at 20 μg/mL, 50 μg/mL, and 100 μg/mL can cause obvious necrosis on leaves, increase active oxygen species (AOS), decrease chlorophyll content, and damage cellular structure. The results enrich the understanding of toxin compounds for <italic>R. solani</italic> and provide valuable insights into the toxicology of <italic>R. solani</italic> AG-3 TB.</p>