AUTHOR=Li Ting , Li Yicun , Wang Jiaqi , Peng Jiashi , Liu Lili , Deng Lichao , Zhang Dawei , Yan Mingli 

TITLE=Expression in A. thaliana and cellular localization reveal involvement of BjNRAMP1 in cadmium uptake

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1261518

DOI=10.3389/fpls.2023.1261518

ISSN=1664-462X

ABSTRACT=<p>Although <italic>Brassica juncea</italic> has demonstrated potential as a hyperaccumulator crop, it was not entirely clear how cadmium (Cd) accumulates in plants. Here, we found that BjNRAMP1 (Natural Resistance-Associated Macrophage Protein 1) plays a crucial role in the accumulation of Cd and manganese (Mn) through its expression in yeast and <italic>Arabidopsis thaliana</italic>. The high concentration of Cd exposure could induce the expression of <italic>BjNRAMP1</italic>. The ectopic expression of <italic>BjNRAMP1</italic> in yeast led to higher accumulation of Cd and Mn compared to the vector control. BjNARAMP1 was localized to the plasma membrane and expressed in the vascular system of roots, leaves, and flowers. The overexpression of <italic>BjNRAMP1</italic> in <italic>A. thaliana</italic> resulted in an increased accumulation of Cd in both roots and shoots, which inhibited the normal growth of transgenic lines. Moreover, Mn uptake in roots was activated by the increase in Cd stress. Together, our results indicated that BjNRAMP1 significantly contributes to the uptake of Mn and Cd in <italic>B. juncea.</italic></p>