AUTHOR=Li Guohui , Manzoor Muhammad Aamir , Wang Guoyu , Chen Cunwu , Song Cheng 

TITLE=Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1258533

DOI=10.3389/fpls.2023.1258533

ISSN=1664-462X

ABSTRACT=<sec><title>Introduction</title><p>KNOX plays a pivotal role in governing plant growth, development, and responses to diverse abiotic and biotic stresses. However, information on the relationship between the <italic>KNOX</italic> gene family and expression levels under different treatments in <italic>Dendrobium</italic> is still limited.</p></sec><sec><title>Methods</title><p>To address this problem, we first used bioinformatics methods and revealed the presence of 19 <italic>KNOX</italic> genes distributed among 13 chromosomes in the <italic>Dendrobium huoshanense</italic> genome. Through an analysis of phylogenetic relationships, these genes were classified into three distinct clades: class I, class II, and class M. Our investigation included promoter analysis, revealing various <italic>cis</italic>-acting elements associated with hormones, growth and development, and abiotic stress responses. Additionally, qRT-PCR experiments were conducted to assess the expression patterns of <italic>DhKNOX</italic> genes under different treatments, including ABA, MeJA, SA, and drought.</p></sec><sec><title>Results</title><p>The results demonstrated differential expression of <italic>DhKNOX</italic> genes in response to these treatments, thereby highlighting their potential roles in stress adaptation.</p></sec><sec><title>Discussion</title><p>Overall, our results contribute important insights for further investigations into the functional characterization of the <italic>Dendrobium KNOX</italic> gene family, shedding light on their roles in plant development and stress responses.</p></sec>