AUTHOR=Morales-Sierra Sarai , Luis Juan Cristo , Jiménez-Arias David , Rancel-Rodríguez Nereida M. , Coego Alberto , Rodriguez Pedro L. , Cueto Mercedes , Borges Andrés A. 

TITLE=Biostimulant activity of Galaxaura rugosa seaweed extracts against water deficit stress in tomato seedlings involves activation of ABA signaling

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1251442

DOI=10.3389/fpls.2023.1251442

ISSN=1664-462X

ABSTRACT=<p>Water scarcity is a serious constraint for agriculture, and global warming and climate change can exacerbate it in many areas. Therefore, sustainable approaches must be implemented to deal with current and future water scarcity scenarios. Genetic and chemical approaches are being applied to manage this limitation and maintain crop yields. In particular, biostimulants obtained from natural sources such as marine algae are promising aids for coping with water deficit stress in agriculture. Here we present a bioprospection study of extracts of the macroalgae <italic>Bonnemaisonia hamifera</italic>, <italic>Galaxaura rugosa</italic>, <italic>Dasycladus vermicularis</italic>, <italic>Ulva clathrata</italic>, <italic>Cystoseira foeniculacea</italic>, <italic>Cystoseira humilis</italic>, <italic>Lobophora dagamae</italic>, <italic>Colpomenia sinuosa</italic> and <italic>Halopteris scoparia</italic> from the north coast of Tenerife, in the Canary Islands. The aqueous extracts of <italic>Bonnemaisonia hamifera</italic>, <italic>Galaxaura rugosa</italic>, <italic>Dasycladus vermicularis and Cystoseira humilis</italic> show biostimulant activity against water deficit stress in tomato seedlings under controlled conditions, providing higher tolerance than the mock-treated control. The <italic>Galaxaura rugosa</italic> extract showed the highest biostimulant activity against water deficit stress. We demonstrate that this positive effect involves the activation of the abscisic acid (ABA) pathway in <italic>Arabidopsis thaliana</italic> (arabidopsis) and <italic>Solanum lycopersicum</italic> (tomato). Application of <italic>G. rugosa</italic> extract to the root system by drenching tomato seedlings subjected to water deficit leads to improved CO<sub>2</sub> assimilation and water use efficiency (WUEp), compared to mock-treated plants. These results highlight a new potential seaweed source of substances with osmoprotectant properties, useful for biostimulant development. Future studies may provide further insight into which components of the seaweed extract induce activation of the ABA pathway.</p>