AUTHOR=Zhang Mingxiong , Rao Yuhui , Chen Xiaofeng , Shi Yunrui , Wei Chonglong , Wang Xianfeng , Wang Lu , Xie Chengjin , Pan Chenglang , Chen Jianming 

TITLE=Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1245555

DOI=10.3389/fpls.2023.1245555

ISSN=1664-462X

ABSTRACT=<p>As an important mangrove species, <italic>Kandelia obovata</italic> plays an irreplaceable role in the coastal ecosystem. However, due to a lack of genetic technology, there is limited research on its functional genes. As such, establishing an efficient and rapid functional verification system is particularly important. In this study,tobacco rattle virus (TRV) and the phytoene desaturase gene <italic>KoPDS</italic> were used as the vector and target gene, respectively, to establish a virus-induced gene silencing system (VIGS) in <italic>K. obovata</italic>. Besides, the system was also used to verify the role of a Chlorophyll a/b binding protein (Cab) gene <italic>KoCAB</italic> in leaf carbon sequestration of <italic>K. obovata.</italic></p><p>RNA-Seq and qRT-PCR showed that the highest gene-silencing efficiency could reach 90% after 10 days of inoculation and maintain above 80% after 15 days, which was achieved with resuspension buffer at pH 5.8 and <italic>Agrobacterium</italic> culture at OD<sub>600</sub> of 0.4-0.6. Taken together, the TRV-mediated VIGS system established herein is the first genetic analysis tool for mangroves, which may greatly impel functional genomics studies in mangrove plants.</p>