AUTHOR=Fu Chun , Liu MingYu 

TITLE=Genome-wide identification and molecular evolution of NAC gene family in Dendrobium nobile

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1232804

DOI=10.3389/fpls.2023.1232804

ISSN=1664-462X

ABSTRACT=<p>NAC transcription factors are an important genes that regulate plant growth and development, and can regulate functions such as fruit ripening in plants. Based on genome data of <italic>Dendrobium nobile</italic>, the <italic>NAC</italic> gene family was identified and analyzed by bioinformatics methods. In this study, we identified 85 <italic>NAC</italic> genes in <italic>Dendrobium nobile</italic> genome, and systematically analyzed the <italic>NAC</italic> gene family. We found that they were distributed unevenly in the nineteen chromosomes. The amino acid length of <italic>D. nobile NAC</italic> gene family (<italic>DnoNACs</italic>) ranged from 80 to 1065, molecular weight ranged from 22.17 to 119.02 kD, and isoelectric point ranged from 4.61~9.26. Its promoter region contains multiple stress responsive elements, including light responsive, gibberellin-responsive, abscisic acid responsiveness, MeJA-responsiveness and drought-inducibility elements. Phylogenetic analysis indicates that the <italic>D. nobile NAC</italic> gene family is most closely related to <italic>Dendrobium catenatum</italic> and <italic>Dendrobium chrysotoxum</italic>. Analysis of SSR loci indicates that the fraction of mononucleotide repeats was the largest, as was the frequency of A/T. Non-coding RNA analysis showed that these 85 <italic>NAC</italic> genes contain 397 miRNAs. The collinearity analysis shows that 9 collinear locis were found on the chromosomes of <italic>D. nobile</italic> with <italic>Arabidopsis thaliana</italic>, and 75 collinear locis with <italic>D.chrysotoxum.</italic> QRT-PCR experiment under different salt concentration and temperature conditions verified the response mechanism of <italic>DnoNAC</italic> gene family under stress conditions. Most <italic>DnoNAC</italic> genes are sensitive to salt stress and temperature stress. The results of this study provide a reference for further understanding the function of <italic>NAC</italic> gene in <italic>D. nobile</italic>.</p>