AUTHOR=Ma Fumin , Shen Yinjuan , Su De , Adnan Muhammad , Wang Maoyao , Jiang Fuhong , Hu Qian , Chen Xiaoru , He Guanyong , Yao Wei , Zhang Muqing , Huang Jiangfeng TITLE=A high-throughput phenotyping assay for precisely determining stalk crushing strength in large-scale sugarcane germplasm JOURNAL=Frontiers in Plant Science VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1224268 DOI=10.3389/fpls.2023.1224268 ISSN=1664-462X ABSTRACT=
Sugarcane is a major industrial crop around the world. Lodging due to weak mechanical strength is one of the main problems leading to huge yield losses in sugarcane. However, due to the lack of high efficiency phenotyping methods for stalk mechanical strength characterization, genetic approaches for lodging-resistant improvement are severely restricted. This study attempted to apply near-infrared spectroscopy high-throughput assays for the first time to estimate the crushing strength of sugarcane stalks. A total of 335 sugarcane samples with huge variation in stalk crushing strength were collected for online NIRS modeling. A comprehensive analysis demonstrated that the calibration and validation sets were comparable. By applying a modified partial least squares method, we obtained high-performance equations that had large coefficients of determination (