AUTHOR=Yang Lingwei , Fang Junchao , Wang Jingxin , Hui Suozhen , Zhou Liang , Xu Bo , Chen Yujuan , Zhang Yuanyuan , Lai Changkai , Jiao Guiai , Sheng Zhonghua , Wei Xiangjin , Shao Gaoneng , Xie Lihong , Wang Ling , Chen Ying , Zhao Fengli , Hu Shikai , Hu Peisong , Tang Shaoqing 

TITLE=Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1222288

DOI=10.3389/fpls.2023.1222288

ISSN=1664-462X

ABSTRACT=<p>3-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for the synthesis of very long-chain fatty acids (VLCFAs) in plants, which determines the carbon chain length of VLCFAs. However, a comprehensive study of KCSs in <italic>Oryza sativa</italic> has not been reported yet. In this study, we identified 22 <italic>OsKCS</italic> genes in rice, which are unevenly distributed on nine chromosomes. The <italic>OsKCS</italic> gene family is divided into six subclasses. Many <italic>cis</italic>-acting elements related to plant growth, light, hormone, and stress response were enriched in the promoters of <italic>OsKCS</italic> genes. Gene duplication played a crucial role in the expansion of the <italic>OsKCS</italic> gene family and underwent a strong purifying selection. Quantitative Real-time polymerase chain reaction (qRT-PCR) results revealed that most <italic>KCS</italic> genes are constitutively expressed. We also revealed that <italic>KCS</italic> genes responded differently to exogenous cadmium stress in <italic>japonica</italic> and <italic>indica</italic> background, and the <italic>KCS</italic> genes with higher expression in leaves and seeds may have functions under cadmium stress. This study provides a basis for further understanding the functions of <italic>KCS</italic> genes and the biosynthesis of VLCFA in rice.</p>