AUTHOR=You Jia , Chen Jingsheng , Hu Yanfeng , Wang Siru , Wang Jianli , Sun Tao , Shen Zhongbao 

TITLE=Identification of cytochrome P450 gene family and functional analysis of HgCYP33E1 from Heterodera glycines

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1219702

DOI=10.3389/fpls.2023.1219702

ISSN=1664-462X

ABSTRACT=<p>The cytochrome P450 (CYP) genes of nematode play a crucial role in the metabolic detoxification of xenobiotics including pesticides. <italic>Heterodera glycines</italic>, also known as the soybean cyst nematode, is a sedentary endoparasite that infests plant roots, causing high annual economic losses in soybean production regions globally. In this study, we identified 36 CYP genes at a genome-wide level of the <italic>H. glycines</italic> isolate TN10 using all CYPs from <italic>Caenorhabditis elegans</italic> as queries. Subsequently, a full-length cDNA of <italic>HgCYP33E1</italic> which was significantly up-regulated by the conventional nematicide abamectin was initially cloned from <italic>H. glycines</italic>. It presented significantly higher expressions in the second-stage juvenile (J2) compared to other parasitic stages of <italic>H. glycines</italic>. qRT-PCR analysis suggested that the expression of <italic>HgCYP33E1</italic> was also xenobiotically induced by soybean root exudate and the metabolites of biocontrol agents. Using RNA interference (RNAi), we investigated the function of <italic>HgCYP33E1</italic> in <italic>H. glycin</italic>e<italic>s</italic> parasitism and nematicide selectivity. Compared to the control and <italic>dsGFP</italic>-treated group, silencing of <italic>HgCYP33E1</italic> did not affect the J2 behaviors and the early invasion ability, while it decreased the number of J4s in soybean roots after 18-d inoculation with the <italic>dsHgCYP33E1</italic>-treated nematodes. In addition, knockdown of <italic>HgCYP33E1</italic> in <italic>H. glycines</italic> resulted in an increase in J2 mortality after 24-h incubation with abamectin compared to the <italic>GFP</italic> dsRNA-soaked and the control group. These findings revealed the potential role of <italic>HgCYP33E1</italic> in the xenobiotic detoxification pathway of <italic>H. glycines</italic>. Moreover, our data also provided valuable gene information for studying the functions of the CYP family in <italic>H. glycines</italic> host adaption.</p>