AUTHOR=Gao Penghua , Qi Ying , Li Lifang , Yang Shaowu , Liu Jiani , Wei Huanyu , Huang Feiyan , Yu Lei 

TITLE=Amorphophallus muelleri activates ferulic acid and phenylpropane biosynthesis pathways to defend against Fusarium solani infection

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1207970

DOI=10.3389/fpls.2023.1207970

ISSN=1664-462X

ABSTRACT=<p><italic>Amorphophallus</italic> sp. is an economically important crop for rural revitalization in southwest China. However, <italic>Fusarium solani</italic> often infects <italic>Amorphophallus</italic> sp. corms during storage, damaging the corm quality and affecting leaf elongation and flowering in the subsequent crop. In this study, the mechanism of resistance to <italic>F. solani</italic> was investigated in the leaf bud and flower bud corms of <italic>Amorphophallus muelleri</italic> through transcriptome and metabolome analyses. A total of 42.52 Gb clean reads and 1,525 metabolites were detected in a total of 12 samples including 3 samples each of disease-free leaf bud corms (LC), leaf bud corms inoculated with <italic>F. solani</italic> for three days (LD), disease-free flower bud corms (FC), and flower bud corms inoculated with <italic>F. solani</italic> for three days (FD). Transcriptome, metabolome, and conjoint analyses showed that ‘MAPK signal transduction’, ‘plant-pathogen interaction’, ‘plant hormone signal transduction’, and other secondary metabolite biosynthesis pathways, including ‘phenylpropane biosynthesis’, ‘arachidonic acid metabolism’, ‘stilbene, diarylheptane and gingerolin biosynthesis’, and ‘isoquinoline alkaloids biosynthesis’, among others, were involved in the defense response of <italic>A. muelleri</italic> to <italic>F. solani</italic>. Ultimately, the expression of six genes of interest (<italic>AmCDPK20</italic>, <italic>AmRBOH</italic>, <italic>AmWRKY33</italic>, <italic>Am4CL</italic>, <italic>Am</italic> POD and <italic>AmCYP73A1</italic>) was validated by real-time fluorescence quantitative polymerase chain reaction, and the results indicated that these genes were involved in the response of <italic>A. muelleri</italic> to <italic>F. solani</italic>. Ferulic acid inhibited the growth of <italic>F. solani</italic>, reducing the harm caused by <italic>F. solani</italic> to <italic>A. muelleri</italic> corms to a certain extent. Overall, this study lays a strong foundation for further investigation of the interaction between <italic>A. muelleri</italic> and <italic>F. solani</italic>, and provides a list of genes for the future breeding of <italic>F. solani</italic>-resistant <italic>A. muelleri</italic> cultivars.</p>