AUTHOR=Yao Wantian , Kong Lingling , Lei Diya , Zhao Bing , Tang Honglan , Zhou Xuan , Lin Yuanxiu , Zhang Yunting , Wang Yan , He Wen , Li Mengyao , Chen Qing , Luo Ya , Wang Xiaorong , Tang Haoru , Zhang Yong 

TITLE=An effective method for establishing a regeneration and genetic transformation system for Actinidia arguta

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1204267

DOI=10.3389/fpls.2023.1204267

ISSN=1664-462X

ABSTRACT=<p>The all-red <italic>A. arguta</italic> (<italic>Actinidia arguta</italic>) is an anthocyanin-rich and excellent hardy fruit. Many studies have focused on the green-fleshed <italic>A. arguta</italic>, and fewer studies have been conducted on the all-red <italic>A. arguta</italic>. Here we reported a regeneration and Agrobacterium-mediated transformation protocol by using leaves of all-red <italic>A. arguta</italic> as explants. Aseptic seedling leaves of <italic>A. arguta</italic> were used as callus-inducing materials. MS medium supplemented with 0.3 mg·L<sup>-1</sup> 2,4-D and 1.0 mg·L<sup>-1</sup> BA was the optimal medium for callus induction of leaves, and medium supplemented with 3 mg·L<sup>-1</sup> tZ and 0.5 mg·L<sup>-1</sup> IAA was optimal for adventitious shoot regeneration. The best proliferation medium for adventitious buds was MS + 1.0 mg·L<sup>-1</sup> BA + 0.3 mg·L<sup>-1</sup> NAA. The best rooting medium was 1/2MS + 0.7 mg·L<sup>-1</sup> IBA with a 100% rooting rate. For the red flesh hardy kiwi variety ‘Purpurna Saduwa’ (<italic>A. arguta</italic> var. <italic>purpurea</italic>), leaves are receptors for Agrobacterium (EHA105)-mediated transformation. The orthogonal experiment was used for the optimization of each genetic transformation parameter and the genetic transformation of the leaves was 21% under optimal conditions. Our study provides technical parameters for applying genetic resources and molecular breeding of kiwifruit with red flesh.</p>