AUTHOR=Yang Su , Chen Jialuo , Ding Yonghe , Huang Qian , Chen Guangna , Ulhassan Zaid , Wei Ji’an , Wang Jian 

TITLE=Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1197781

DOI=10.3389/fpls.2023.1197781

ISSN=1664-462X

ABSTRACT=<p>The <italic>Brassica napus</italic> (<italic>B. napus</italic>) <italic>LOR</italic> (<italic>Lurp-One-Related</italic>) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in <italic>Arabidopsis</italic> showed that <italic>LOR</italic> family members played important roles in <italic>Hyaloperonospora parasitica</italic> (<italic>Hpa</italic>) defense. Nevertheless, there is a paucity of research investigating the role of the <italic>LOR</italic> gene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56 <italic>LOR</italic> genes in <italic>B. napus</italic>, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56 <italic>BnLORs</italic> could be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56 <italic>BnLOR</italic> members have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated that <italic>BnLORs</italic> involved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern of <italic>BnLOR</italic> family members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validate <italic>BnLOR</italic> gene expression under temperature, salinity and ABA stress, revealing that most <italic>BnLORs</italic> showed inducibility. This study enhanced our comprehension of the <italic>B. napus LOR</italic> gene family and could provide valuable information for identifying and selecting genes for stress resistant breeding.</p>