AUTHOR=Li Jia , Cai Bo , Chang Sheng , Yang Ying , Zi Shuhui , Liu Tao 

TITLE=Mechanisms associated with the synergistic induction of resistance to tobacco black shank in tobacco by arbuscular mycorrhizal fungi and β-aminobutyric acid

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1195932

DOI=10.3389/fpls.2023.1195932

ISSN=1664-462X

ABSTRACT=<p>Tobacco black shank (TBS), caused by <italic>Phytophthora nicotianae</italic>, is one of the most harmful diseases of tobacco. There are many studies have examined the mechanism underlying the induction of disease resistance by arbuscular mycorrhizal fungi (AMF) and β-aminobutyric acid (BABA) alone, but the synergistic effects of AMF and BABA on disease resistance have not yet been studied. This study examined the synergistic effects of BABA application and AMF inoculation on the immune response to TBS in tobacco. The results showed that spraying BABA on leaves could increase the colonization rate of AMF, the disease index of tobacco infected by <italic>P.nicotianae</italic> treated with AMF and BABA was lower than that of <italic>P.nicotianae</italic> alone. The control effect of AMF and BABA on tobacco infected by <italic>P.nicotianae</italic> was higher than that of AMF or BABA and <italic>P.nicotianae</italic> alone. Joint application of AMF and BABA significantly increased the content of N, P, and K in the leaves and roots, in the joint AMF and BABA treatment than in the sole <italic>P. nicotianae</italic> treatment. The dry weight of plants treated with AMF and BABA was 22.3% higher than that treated with <italic>P.nicotianae</italic> alone. In comparison to <italic>P. nicotianae</italic> alone, the combination treatment with AMF and BABA had increased Pn, Gs, Tr, and root activity, while <italic>P. nicotianae</italic> alone had reduced Ci, H<sub>2</sub>O<sub>2</sub> content, and MDA levels. SOD, POD, CAT, APX, and <italic>Ph</italic> activity and expression levels were increased under the combined treatment of AMF and BABA than in <italic>P.nicotianae</italic> alone. In comparison to the treatment of <italic>P.nicotianae</italic> alone, the combined use of AMF and BABA increased the accumulation of GSH, proline, total phenols, and flavonoids. Therefore, the joint application of AMF and BABA can enhance the TBS resistance of tobacco plants to a greater degree than the application of either AMF or BABA alone. In summary, the application of defense-related amino acids, combined with inoculation with AMF, significantly promoted immune responses in tobacco. Our findings provide new insights that will aid the development and use of green disease control agents.</p>