AUTHOR=Hu Ruolin , Wang Jiali , Yang Huiqing , Wei Dayong , Tang Qinglin , Yang Yang , Tian Shibing , Wang Zhimin 

TITLE=Comparative transcriptome analysis reveals the involvement of an MYB transcriptional activator, SmMYB108, in anther dehiscence in eggplant

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1164467

DOI=10.3389/fpls.2023.1164467

ISSN=1664-462X

ABSTRACT=<p>Male sterility is a highly attractive agronomic trait as it effectively prevents self-fertilization and facilitates the production of high-quality hybrid seeds in plants. Timely release of mature pollen following anther dehiscence is essential for stamen development in flowering plants. Although several theories have been proposed regarding this, the specific mechanism of anther development in eggplant remains elusive. In this study, we selected an R2R3-MYB transcription factor gene, <italic>SmMYB108</italic>, that encodes a protein localized primarily in the nucleus by comparing the transcriptomics of different floral bud developmental stages of the eggplant fertile line, F142. Quantitative reverse transcription polymerase chain reaction revealed that <italic>SmMYB108</italic> was preferentially expressed in flowers, and its expression increased significantly on the day of flowering. Overexpression of <italic>SmMYB108</italic> in tobacco caused anther dehiscence. In addition, we found that <italic>SmMYB108</italic> primarily functions as a transcriptional activator <italic>via</italic> C-terminal activation (amino acid 262–317). Yeast one-hybrid and dual-luciferase reporter assays revealed that genes (<italic>SmMYB21</italic>, <italic>SmARF6</italic>, and <italic>SmARF8</italic>) related to anther development targeted the <italic>SmMYB108</italic> promoter. Overall, our results provide insights into the molecular mechanisms involved in the regulation of anther development by <italic>SmMYB108</italic>.</p>