AUTHOR=Yang Min , Hou GouYan , Peng YuTing , Wang LiangXin , Liu XiaoYang , Jiang YuYan , He CaiXia , She MuSha , Zhao ManTong , Chen Qing , Li Mengyao , Zhang Yong , Lin Yuanxiu , Zhang Yunting , Wang Yan , He Wen , Wang Xiaorong , Tang Haoru , Luo Ya 

TITLE=FaGAPC2/FaPKc2.2 and FaPEPCK reveal differential citric acid metabolism regulation in late development of strawberry fruit

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1138865

DOI=10.3389/fpls.2023.1138865

ISSN=1664-462X

ABSTRACT=<p>Citric acid is the primary organic acid that affects the taste of strawberry fruit. Glycolysis supplies key substrates for the tricarboxylic acid cycle (TCA cycle). However, little is known about the regulatory mechanisms of glycolytic genes on citric acid metabolism in strawberry fruits. In this study, the citric acid content of strawberry fruit displayed a trend of rising and decreasing from the initial red stage to the full red stage and then dark red stage. Thus, a difference in citric acid metabolic regulation was suspected during strawberry fruit development. In addition, overexpression of either cytoplasm glyceraldehyde-3-phosphate dehydrogenase (FxaC_14g13400, namely <italic>FaGAPC2</italic>) or pyruvate kinase (FxaC_15g00080, namely <italic>FaPKc2.2</italic>) inhibited strawberry fruit ripening and the accumulation of citric acid, leading to a range of maturity stages from partial red to full red stage. The combined transcriptome and metabolome analysis revealed that overexpression of <italic>FaGAPC2</italic> and <italic>FaPKc2.2</italic> significantly suppressed the expression of phosphoenolpyruvate carboxykinase (FxaC_1g21491, namely <italic>FaPEPCK</italic>) but enhanced the content of glutamine and aspartic acid. Meanwhile, the activities of PEPCK and glutamate decarboxylase (GAD) were inhibited, but the activities of glutamine synthase (GS) were increased in <italic>FaGAPC2/FaPKc2.2</italic>-overexpressed fruit. Further, functional verification demonstrated that overexpression of <italic>FaPEPCK</italic> can promote strawberry fruit ripening, resulting in a range of maturity stage from full red to dark red stage, while the citric acid synthase (CS) activities and citric acid content were significantly decreased. Overall, this study revealed that <italic>FaGAPC2</italic>/<italic>FaPKc2.2</italic> and <italic>FaPEPCK</italic> perform an important role in reducing citric acid content in strawberry fruit, and <italic>FaGAPC2</italic>/<italic>FaPKc2.2</italic> mainly by promoting the GS degradation pathway and <italic>FaPEPCK</italic> mainly by inhibiting the CS synthesis pathway.</p>