AUTHOR=Chen Na , Pan Lijuan , Yang Zhen , Su Maowen , Xu Jing , Jiang Xiao , Yin Xiangzhen , Wang Tong , Wan Feifei , Chi Xiaoyuan 

TITLE=A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1136626

DOI=10.3389/fpls.2023.1136626

ISSN=1664-462X

ABSTRACT=<p>Abiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene <italic>AhMYB30</italic> obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicated that the overexpression of <italic>AhMYB30</italic> in <italic>Arabidopsis</italic> could enhance the resistance of transgenic plants to freezing and salt stresses. The expression of stress-response genes <italic>RD29A</italic> (<italic>Response-to-Dehydration 29A</italic>), <italic>COR15A</italic> (<italic>Cold-Regulated 15A</italic>), <italic>KIN1</italic> (<italic>Kinesin 1</italic>) and <italic>ABI2</italic> (<italic>Abscisic acid Insensitive 2</italic>) increased in transgenic plants compared with in wild-type. Subcellular localization and transcriptional autoactivation validation demonstrated that AhMYB30 has essential features of transcription factors. Therefore, AhMYB30 may increase salt and freezing stress tolerance as the transcription factor (TF) in <italic>Arabidopsis</italic> through both DREB/CBF and ABA-signaling pathways. Our results lay the theoretical foundation for exploring stress resistance mechanisms of peanut and offering novel genetic resources for molecular breeding.</p>