AUTHOR=Lu Qinghua , Luo Xiangwen , Yang Xiao , Zhou Tong , Zhang Yu , Lan Ying , Zhang Deyong , Zheng Limin , Li Yixin , Li Li , Zhang Songbai , Liu Yong 

TITLE=CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1122978

DOI=10.3389/fpls.2023.1122978

ISSN=1664-462X

ABSTRACT=<p>Vacuolar ATPases (V-ATPases) are proton pumps for proton translocation across membranes that utilize energy derived from ATP hydrolysis; OsV-ATPase subunit d (OsV-ATPase d) is part of an integral, membrane-embedded V0 complex in the V-ATPase complex. Whether OsV-ATPase d is involved in phytohormone biosynthesis and resistance in rice remains unknown. The knockout mutants of <italic>OsV-ATPase d</italic> in rice were generated using the CRISPR/Cas9 system, and mutation of <italic>OsV-ATPase d</italic> did not show any detrimental effect on plant growth or yield productivity. Transcriptomic results showed that <italic>OsV-ATPase d</italic> is probably involved in mediating the biosynthesis of plant hormones and resistance in rice. Compared to wild type, mutation of <italic>OsV-ATPase d</italic> significantly increased JA and ABA biosynthesis and resistance against <italic>Southern rice black-streaked dwarf virus</italic> (SRBSDV), but it decreased resistance against <italic>Rice stripe virus</italic> (RSV) in rice. The data presented in this study reveal that <italic>OsV-ATPase d</italic> mediates phytohormone biosynthesis and virus resistance in rice and can be selected as a potential target for resistance breeding in rice.</p>