AUTHOR=Xu Juanjuan , Yang Caiyu , Ji Shangyao , Ma Hui , Lin Jingwei , Li Hui , Chen Shuisen , Xu Hai , Zhong Ming 

TITLE=Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1118548

DOI=10.3389/fpls.2023.1118548

ISSN=1664-462X

ABSTRACT=<sec><title>Introduction</title><p>β-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied.</p></sec><sec><title>Methods</title><p>Based on cloned the <italic>mannanase (MAN)</italic> gene from <italic>Mirabilis jalapa</italic> L., the study was carried out by heterologously expressing the gene in <italic>Arabidopsis thaliana</italic>, and then observing the plant phenotypes and measuring relevant physiological and biochemical indicators under 150 mM salt treatment.</p></sec><sec><title>Results and discussion</title><p>The results indicate that MirMAN is a protein with a glycohydrolase-specific structural domain located in the cell wall. We first found that MirMAN reduced the susceptibility of transgenic <italic>Arabidopsis thaliana</italic> to high salt stress and increased the survival rate of plants by 38%. This was corroborated by the following significant changes, including the reduction in reactive oxygen species (ROS) levels, increase in antioxidant enzyme activity, accumulation of soluble sugars and increase of the expression level of RD29 in transgenic plants. We also found thatthe heterologous expression of <italic>MirMAN</italic> promoted root growth mainly by elongating the primary roots and increasing the density of lateral roots. Meanwhile, the expression of <italic>ARF7, ARF19, LBD16</italic> and <italic>LBD29</italic> was up-regulated in the transgenic plants, and the concentration of IAA in the roots was increased. Those results indicate that <italic>MirMAN</italic> is involved in the initiation of lateral root primordia in transgenic plants through the <italic>IAA-ARF</italic> signalling pathway. In conclusion, MirMAN improves plant salt tolerance not only by regulating ROS homeostasis, but also by promoting the development of lateral roots. Reflecting the potential of the <italic>MirMAN</italic> to promote root plastic development in adaptation to salt stress adversity.</p></sec>