AUTHOR=Santillán Martínez Miguel I. , Gao Dongli , Appiano Michela , Derks Inge , Huibers Robin P. , Spil Glenn , Wang Xulan , Visser Richard G. F. , Wolters Anne-Marie A. , Bai Yuling 

TITLE=ZED1-related kinase 13 is required for resistance against Pseudoidium neolycopersici in Arabidopsis accession Bla-6

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1111322

DOI=10.3389/fpls.2023.1111322

ISSN=1664-462X

ABSTRACT=<p>To explore specific components of resistance against the tomato-adapted powdery mildew pathogen <italic>Pseudoidium neolycopersici</italic> (<italic>On</italic>) in the model plant Arabidopsis, we performed a disease assay in 123 accessions. When testing the resistance in the F<sub>1</sub> from crossings between resistant accessions with susceptible Col-0 or Sha, only the progeny of the cross between accession Bla-6 and Col-0 displayed a completely resistant phenotype. The resistance in Bla-6 is known to be specific for <italic>Pseudoidium neolycopersici</italic>. QTL analysis and fine-mapping through several rounds of recombinant screenings allowed us to locate a major resistance QTL in an interval on chromosome 1, containing two candidate genes and an intergenic insertion. <italic>Via</italic> CRISPR/Cas9 targeted mutagenesis, we could show that knocking out the <italic>ZED-1 RELATED KINASE 13</italic> (<italic>ZRK13</italic>) gene compromised the <italic>On</italic> resistance in Bla-6. Several polymorphisms are observed in the ZRK13 allelic variant of Bla-6 when compared to the Col-0 protein.</p>