AUTHOR=Dobbs J. T. , Kim M.-S. , Reynolds G. J. , Wilhelmi N. , Dumroese R. K. , Klopfenstein N. B. , Fraedrich S. W. , Cram M. M. , Bronson J. , Stewart J. E. 

TITLE=Fusarioid community diversity associated with conifer seedlings in forest nurseries across the contiguous USA

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1104675

DOI=10.3389/fpls.2023.1104675

ISSN=1664-462X

ABSTRACT=<sec><title>Introduction</title><p>Fusarioid fungi that cause damping-off and root diseases can result in significant losses to conifer crops produced in forest nurseries across the USA. These nurseries are vital to reforestation and forest restoration efforts. Understanding the diversity of Fusarioid fungi associated with damping-off and root diseases of conifer seedlings can provide an approach for targeted management techniques to limit seedling losses and pathogen spread to novel landscapes.</p></sec><sec><title>Methods</title><p>This study identifies 26 <italic>Fusarium</italic> spp. (<italic>F. acuminatum</italic>, <italic>F. annulatum</italic>, <italic>F. avenaceum</italic>, <italic>F. brachygibbosum</italic>, <italic>F. clavus</italic>, <italic>F. commune</italic>, <italic>F. cugenangense</italic>, <italic>F. diversisporum</italic>, <italic>F. elaeagni</italic>, <italic>F. elaeidis</italic>, <italic>F. flocciferum</italic>, <italic>F. fredkrugeri</italic>, <italic>F. fujikuroi</italic>, <italic>F. grosmichelii</italic>, <italic>F. ipomoeae</italic>, <italic>F. lactis</italic>, <italic>F. languescens</italic>, <italic>F. luffae</italic>, <italic>F. odoratissimum</italic>, <italic>F. oxysporum</italic>, <italic>F. queenslandicum</italic>, <italic>F. redolens</italic>, <italic>F. torulosum</italic>, <italic>F. triseptatum</italic>, <italic>F. vanleeuwenii</italic>, &amp; <italic>F. verticillioides</italic>), 15 potential species within <italic>Fusarium</italic> and <italic>Neocosmospora</italic> species complexes (two from <italic>F. fujikuroi</italic> species complex, nine from <italic>F. oxysporum</italic> species complex, three from <italic>F. tricinctum</italic> species complex, and one from <italic>Neocosmospora</italic> species complex), and four <italic>Neocosmospora</italic> spp. (<italic>N. falciforme</italic>, <italic>N. metavorans</italic>, <italic>N. pisi</italic>, &amp; <italic>N. solani</italic>) and associated host information collected from conifer-producing nurseries across the contiguous USA.</p></sec><sec><title>Results</title><p>Phylogenetic analyses identified Fusarioid fungi haplotypes that were associated with 1) host specificity, 2) localization to geographic regions, or 3) generalists found on multiple hosts across diverse geographic regions.</p></sec><sec><title>Discussion</title><p>The haplotypes and novel species identified on conifer seedlings should be considered for further analysis to determine pathogenicity, pathogen spread, and assess management practices.</p></sec>