AUTHOR=Wang Mengxue , Luo Shuwei , Fan Baofang , Zhu Cheng , Chen Zhixiang 

TITLE=LIP5, a MVB biogenesis regulator, is required for rice growth

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1103028

DOI=10.3389/fpls.2023.1103028

ISSN=1664-462X

ABSTRACT=<p>LYST-INTERACTING PROTEIN5 (LIP5) is a conserved regulator of multivesicular body (MVB) biogenesis in eukaryotes. In Arabidopsis, AtLIP5 is a target of stress-responsive MITOGEN-ACTIVATED PROTEIN KINASE3 and 6 and mediates stress-induced MVB biogenesis to promote stress responses. However, Arabidopsis <italic>atlip5</italic> knockout mutants are normal in growth and development. Here we report that rice <italic>OsLIP5</italic> gene could fully restore both the disease resistance and salt tolerance of the Arabidopsis <italic>oslip5</italic> mutant plants to the wild-type levels. Unlike Arabidopsis <italic>atlip5</italic> mutants, rice <italic>oslip5</italic> mutants were severely stunted, developed necrotic lesions and all died before flowering. Unlike in Arabidopsis, LIP5 regulated endocytosis under both stress and normal conditions in rice. These findings indicate that there is strong evolutionary divergence among different plants in the role of the conserved LIP5-regulated MVB pathway in normal plant growth.</p>