AUTHOR=Zhao Xijuan , Zhang Huiling , Liu Tengfei , Zhao Yanan , Hu Xinxi , Liu Shengxuan , Lin Yuan , Song Botao , He Changzheng 

TITLE=Transcriptome analysis provides StMYBA1 gene that regulates potato anthocyanin biosynthesis by activating structural genes

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1087121

DOI=10.3389/fpls.2023.1087121

ISSN=1664-462X

ABSTRACT=<p>Anthocyanin biosynthesis is affected by light, temperature, and other environmental factors. The regulation mode of light on anthocyanin synthesis in apple, pear, tomato and other species has been reported, while not clear in potato. In this study, potato RM-210 tubers whose peel will turn purple gradually after exposure to light were selected. Transcriptome analysis was performed on RM-210 tubers during anthocyanin accumulation. The expression of <italic>StMYBA1</italic> gene continued to increase during the anthocyanin accumulation in RM-210 tubers. Moreover, co-expression cluster analysis of differentially expressed genes showed that the expression patterns of <italic>StMYBA1</italic> gene were highly correlated with structural genes <italic>CHS</italic> and <italic>CHI</italic>. The promoter activity of <italic>StMYBA1</italic> was significantly higher in light conditions, and StMYBA1 could activate the promoter activity of structural genes <italic>StCHS</italic>, <italic>StCHI</italic>, and <italic>StF3H</italic>. Further gene function analysis found that overexpression of <italic>StMYBA1</italic> gene could promote anthocyanin accumulation and structural gene expression in potato leaves. These results demonstrated that <italic>StMYBA1</italic> gene promoted potato anthocyanin biosynthesis by activating the expression of structural genes under light conditions. These findings provide a theoretical basis and genetic resources for the regulatory mechanism of potato anthocyanin synthesis.</p>