AUTHOR=Guden Birgul , Yol Engin , Erdurmus Cengiz , Lucas Stuart James , Uzun Bulent 

TITLE=Construction of a high-density genetic linkage map and QTL mapping for bioenergy-related traits in sweet sorghum [Sorghum bicolor (L.) Moench]

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1081931

DOI=10.3389/fpls.2023.1081931

ISSN=1664-462X

ABSTRACT=<p>Sorghum is an important but arguably undervalued cereal crop, grown in large areas in Asia and Africa due to its natural resilience to drought and heat. There is growing demand for sweet sorghum as a source of bioethanol as well as food and feed. The improvement of bioenergy-related traits directly affects bioethanol production from sweet sorghum; therefore, understanding the genetic basis of these traits would enable new cultivars to be developed for bioenergy production. In order to reveal the genetic architecture behind bioenergy-related traits, we generated an F<sub>2</sub> population from a cross between sweet sorghum cv. ‘Erdurmus’ and grain sorghum cv. ‘Ogretmenoglu’. This was used to construct a genetic map from SNPs discovered by double-digest restriction-site associated DNA sequencing (ddRAD-seq). F<sub>3</sub> lines derived from each F<sub>2</sub> individual were phenotyped for bioenergy-related traits in two different locations and their genotypes were analyzed with the SNPs to identify QTL regions. On chromosomes 1, 7, and 9, three major plant height (PH) QTLs (<italic>qPH</italic>1.1, <italic>qPH</italic>7.1, and <italic>qPH</italic>9.1) were identified, with phenotypic variation explained (PVE) ranging from 10.8 to 34.8%. One major QTL (<italic>qPJ</italic>6.1) on chromosome 6 was associated with the plant juice trait (PJ) and explained 35.2% of its phenotypic variation. For fresh biomass weight (FBW), four major QTLs (<italic>qFBW</italic>1.1, <italic>qFBW</italic>6.1, <italic>qFBW</italic>7.1, and <italic>qFBW</italic>9.1) were determined on chromosomes 1, 6, 7, and 9, which explained 12.3, 14.5, 10.6, and 11.9% of the phenotypic variation, respectively. Moreover, two minor QTLs (<italic>qBX</italic>3.1 and <italic>qBX</italic>7.1) of Brix (BX) were mapped on chromosomes 3 and 7, explaining 8.6 and 9.7% of the phenotypic variation, respectively. The QTLs in two clusters (<italic>qPH</italic>7.1/<italic>qBX</italic>7.1 and <italic>qPH</italic>7.1/<italic>qFBW</italic>7.1) overlapped for PH, FBW and BX. The QTL, <italic>qFBW</italic>6.1, has not been previously reported. In addition, eight SNPs were converted into cleaved amplified polymorphic sequences (CAPS) markers, which can be easily detected by agarose gel electrophoresis. These QTLs and molecular markers can be used for pyramiding and marker-assisted selection studies in sorghum, to develop advanced lines that include desirable bioenergy-related traits.</p>