AUTHOR=Tang Yanhong , Yang Xiaoyue , Li Han , Shuai Yating , Chen Wang , Ma Dongfang , Lü Zhichuang 

TITLE=Uncovering the role of wheat magnesium transporter family genes in abiotic responses

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1078299

DOI=10.3389/fpls.2023.1078299

ISSN=1664-462X

ABSTRACT=<sec><title>Background</title><p>The CorA / MGT / MRS2 family proteins are an important group of magnesium transporter proteins that maintain magnesium ion homeostasis in plant cells. However, little is known about the MGT functions in wheat.</p></sec><sec><title>Methods</title><p>The known MGT sequences were used as queries to BlastP against wheat genome IWGSC RefSeq v2.1 assembly (E-value &lt;10–5). Chromosome localization information for each <italic>TaMGT</italic> gene was obtained from the GFF3 file of the wheat genome data (IWGSCv2.1).The sequence of 1500 bp upstream of the <italic>TaMGT</italic> genes was extracted from the wheat genome data. The cis-elements were analyzed using PlantCARE online tool.</p></sec><sec><title>Result</title><p>A total of 24 <italic>MGT</italic> genes were identified on 18 chromosomes of wheat. After functional domain analysis, only <italic>TaMGT1A</italic>, <italic>TaMGT1B</italic>, and <italic>TaMGT1D</italic> had GMN mutations to AMN, while all the other genes had conserved GMN tripeptide motifs. Expression profiling showed that the <italic>TaMGT</italic> genes were differentially expressed under different stresses and at different growth and development stages. The expression levels of <italic>TaMGT4B</italic> and <italic>TaMGT4A</italic> were significantly up-regulated in cold damage. In addition, qRT-PCR results also confirmed that these <italic>TaMGT</italic> genes are involved in the wheat abiotic stress responses.</p></sec><sec><title>Conclusion</title><p>In conclusion, The results of our research provide a theoretical basis for further research on the function of <italic>TaMGT</italic> gene family in wheat.</p></sec>