AUTHOR=Sharma Anuj , Li Jian , Wente Rebecca , Minsavage Gerald V. , Gill Upinder S. , Ortega Arturo , Vallejos C. Eduardo , Hart John P. , Staskawicz Brian J. , Mazourek Michael R. , Stall Robert E. , Jones Jeffrey B. , Hutton Samuel F. 

TITLE=Mapping of the bs5 and bs6 non-race-specific recessive resistances against bacterial spot of pepper

JOURNAL=Frontiers in Plant Science

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1061803

DOI=10.3389/fpls.2023.1061803

ISSN=1664-462X

ABSTRACT=<p>Bacterial spot caused by <italic>Xanthomonas euvesicatoria</italic> is a major disease of pepper (<italic>Capsicum annuum</italic> L.) in warm and humid production environments. Use of genetically resistant cultivars is an effective approach to manage bacterial spot. Two recessive resistance genes, <italic>bs5</italic> and <italic>bs6</italic>, confer non-race-specific resistance against bacterial spot. The objective of our study was to map these two loci in the pepper genome. We used a genotyping-by-sequencing approach to initially map the position of the two resistances. Segregating populations for <italic>bs5</italic> and <italic>bs6</italic> were developed by crossing susceptible Early CalWonder (ECW) with near-isogenic lines ECW50R (<italic>bs5</italic> introgression) or ECW60R (<italic>bs6</italic> introgression). Following fine-mapping, <italic>bs5</italic> was delimited to a ~535 Kbp interval on chromosome 3, and <italic>bs6</italic> to a ~666 Kbp interval in chromosome 6. We identified 14 and 8 candidate resistance genes for <italic>bs5</italic> and <italic>bs6</italic>, respectively, based on predicted protein coding polymorphisms between ECW and the corresponding resistant parent. This research enhances marker-assisted selection of <italic>bs5</italic> and <italic>bs6</italic> in breeding programs and is a crucial step towards elucidating the molecular mechanisms underlying the resistances.</p>