AUTHOR=Zhang Hui , Li Zefeng , Xu Guoyun , Bai Ge , Zhang Peipei , Zhai Niu , Zheng Qingxia , Chen Qiansi , Liu Pingping , Jin Lifeng , Zhou Huina 

TITLE=Genome-wide identification and characterization of NPF family reveals NtNPF6.13 involving in salt stress in Nicotiana tabacum

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.999403

DOI=10.3389/fpls.2022.999403

ISSN=1664-462X

ABSTRACT=<p>Proteins of the Nitrate Transporter 1/Peptide Transporter (NPF) family transport a diverse variety of substrates, such as nitrate, peptides, hormones and chloride. In this study, a systematic analysis of the tobacco (<italic>Nicotiana tabacum</italic>) <italic>NPF</italic> family was performed in the cultivated ‘K326’. In total, 143 <italic>NtNPF</italic> genes were identified and phylogenetically classified into eight subfamilies, NPF1 to NPF8, based on the classification of NPF families in other plant species. The chromosomal locations and structures of the <italic>NtNPF</italic> genes were analyzed. The expression profiles of <italic>NtNPF</italic> genes under NaCl stress were analyzed to screen the possible <italic>NPF</italic> genes involving in chloride regulation in tobacco. Most <italic>NtNPF6</italic> genes responded to salt stress in the roots and leaves. The expression of <italic>NtNPF6.13</italic> was significantly down-regulated after salt stress for 12h. The chloride content was reduced in the roots of <italic>ntnpf6.13</italic> mutant. These findings support the participation of <italic>NtNPF6.13</italic> in chloride uptake. Several other <italic>NtNPF</italic> genes that play potential roles in chloride metabolism of tobacco require further study.</p>