AUTHOR=Zhai Lu , Xie Lingli , Xu JinSong , Xu Benbo , Dong Jing , Zhang XueKun 

TITLE=Study on exogenous application of thidiazuron on seed size of Brassica napus L.

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.998698

DOI=10.3389/fpls.2022.998698

ISSN=1664-462X

ABSTRACT=<p>Thidiazuron (TDZ) is a novel and efficient cytokinin commonly used in tissue culture, and numerous studies have demonstrated that TDZ can increase berry size. However, no study to date has explored the effect of TDZ on seed size of <italic>Brassica napus</italic> and the mechanism. To shed light on the effect of TDZ on the seed size of <italic>B. napus</italic>, four different concentrations of TDZ were applied to <italic>B. napus</italic>. Results indicated that TDZ treatment could increase the seed diameter and silique length of <italic>B. napus</italic> to varying degrees and 100 and 200 μmol/L TDZ treatments were the most effective with a 3.6 and 4.6% increase in seed diameter, respectively. In addition, the yield of <italic>B. napus</italic> was also substantially increased under TDZ treatment. On the other hand, confocal micrographs of embryos and cotyledon cells suggested that embryos and their cotyledon epidermal cells treated with 200 μmol/L TDZ were obviously larger in size than the control. Furthermore, TDZ promoted the upregulation of some key maternal tissue growth-related genes, including two G-protein signaling genes (<italic>AGG3</italic> and <italic>RGA1</italic>) and two transcriptional regulators (<italic>ANT</italic> and <italic>GS2</italic>). The expression analysis of genes related to the auxin metabolic pathways, G-protein signaling, endosperm growth and transcriptional regulators confirmed that treatment with TDZ negatively regulated the key genes <italic>ABI5</italic>, <italic>AGB1</italic>, <italic>AP2</italic>, <italic>ARF2</italic>, and <italic>ARF18</italic> during bud development stage and florescence. The results strongly suggested that TDZ might regulate the transcriptional levels of key genes involved in auxin metabolic pathways, G-protein signaling, endosperm growth and transcriptional regulators, which resulted in bigger cotyledon epidermal cells and seed size in <italic>B. napus</italic>. This study explored the mechanism of TDZ treatment on the seed size of <italic>B. napus</italic> and provided an important reference for improving rapeseed yield.</p>