AUTHOR=Wang Yiping , Cui Ying , Liu Bin , Wang Ying , Sun Shaoying , Wang Jingwen , Tan Mengmeng , Yan Hao , Zhang Yanni 

TITLE=Lilium pumilum stress-responsive NAC transcription factor LpNAC17 enhances salt stress tolerance in tobacco

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.993841

DOI=10.3389/fpls.2022.993841

ISSN=1664-462X

ABSTRACT=<p><italic>Lilium pumilum</italic> is a perennial herb with ornamental edible and medicinal value. It is an excellent wild germplasm resource with wide distribution and strong resistance. The NAC family of transcription factors is unique to higher plants. The NAC family plays a regulatory role in plant growth and development and participates in plant responses to biotic and abiotic stresses. The <italic>LpNAC17</italic> gene of <italic>L. pumilum</italic> was cloned and transformed into tobacco to investigate the response of transgenic tobacco to salt stress. The results showed that the net photosynthetic rate and contents of chlorophyll in <italic>LpNAC17</italic> over-expressed tobacco were higher than those in the control plants, while the stomatal conductance, transpiration rate and intercellular CO<sub>2</sub> concentration were lower than those in the controls. The activity of superoxide dismutase, peroxidase, catalase, and the content of proline in <italic>LpNAC17</italic> over-expressed tobacco were higher than those in the controls, while the content of malondialdehyde, superoxide anion, and hydrogen peroxide were lower than that in the control. Nitro-blue tetrazolium staining and 3,3′-diaminobenzidine tissue localization showed that the contents of <inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mrow><mml:mtext>O</mml:mtext></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow><mml:mrow><mml:mo>-</mml:mo></mml:mrow></mml:msubsup></mml:math></inline-formula> and H<sub>2</sub>O<sub>2</sub> in transgenic tobacco was lower than in the controls. The expression levels of <italic>NtSOD, NtPOD, NtCAT, NtHAK1, NtPMA4</italic>, and <italic>NtSOS1</italic> in the transgenic tobacco were higher than those in the controls. Therefore, this study provides a gene source for molecular breeding of salt-tolerant plants through genetic engineering, and lays a foundation for further research on salt-tolerant Lily.</p>