AUTHOR=Liang Jiayu , Zou Rong , Huang Yang , Qin Huizhen , Tang Jianmin , Wei Xiao , Liang Yu , Chai Shengfeng 

TITLE=Structure and diversity of mycorrhizal fungi communities of different part of Bulbophyllum tianguii in three terrestrial environments

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.992184

DOI=10.3389/fpls.2022.992184

ISSN=1664-462X

ABSTRACT=<p>Mycorrhizal fungi plays important roles in the seed germination and subsequent growth of orchids. The research of fungi in orchid roots, especially dominant mycorrhizal fungi is critical for orchids protection. In this study, the fungal community and composition of mycorrhizal fungi in roots, rhizomes and rhizosphere soil of <italic>Bulbophyllum tianguii</italic> grown in three terrestrial environments were analyzed by the second generation sequencing technology. The results of OTU clustering and α and β diversity analysis showed that there were significant differences in fungal communities in roots, rhizomes and rhizosphere soil of <italic>B. tianguii</italic>. The total number of OTUs in rhizomes was much less than that in roots and rhizosphere soil. The number of OTUs in rhizosphere soil and the diversity of mycorrhizal fungi were the highest. Meanwhile, the species and abundance of mycorrhizal fungi in roots and rhizomes of <italic>B. tianguii</italic> were different from those in rhizosphere soil. For different elevations, compared with <italic>B. tianguii</italic> that grow in middle of Tiankeng and top of Tiankeng, the OTUs number of <italic>B. tianguii</italic> in orchid garden is richest, and the diversity of mycorrhizal fungi in orchid garden was significantly higher than other locations. Among the three different habitats of <italic>B. tianguii</italic>, the number of OTUs in humus soil and stone habitats was notably higher than tree habitats, and the diversity of mycorrhizal fungi in humus soil was the highest. The analysis of mycorrhizal fungi in different habitats and altitudes of <italic>B. tianguii</italic> showed that Sebacina and Exophiala were the dominant mycorrhizal fungi in <italic>B. tianguii</italic>. The results of species annotation, phylogenetic tree and co-occurrence network analysis showed the dominant mycorrhizal fungi of <italic>B. tianguii</italic> mainly included Sebacina, Cladosporium, Exophiala, Fusarium. This study reveals the symbiotic relationship between Sebacina, Exophiala, Cladosporium and the <italic>B. Tianguii</italic>. It will provide a theoretical basis for the protection and biological function study of <italic>B. Tianguii</italic>.</p>