AUTHOR=Yan Peng , Yu Jiawen , Fang Xinmei , Li Shuying , Han Shan , Lin Tiantian , Liu Yinggao , Yang Chunlin , He Fang , Zhu Tianhui , Li Shujiang 

TITLE=Identification of the interacting proteins of Bambusa pervariabilis × Dendrocalamopsis grandis in response to the transcription factor ApCtf1β in Arthrinium phaeospermum

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.991077

DOI=10.3389/fpls.2022.991077

ISSN=1664-462X

ABSTRACT=<p><italic>Arthrinium phaeospermum</italic> is the main pathogen that causes <italic>Bambusa pervariabilis × Dendrocalamopsis grandis</italic> blight. It secretes the cutinase transcription factor <italic>ApCtf1β</italic>, which has been shown to play an important role in <italic>B. pervariabilis × D. grandis</italic> virulence. However, knowledge about the interaction target genes of <italic>ApCtf1β</italic> in <italic>B. pervariabilis × D. grandis</italic> remains limited. A cDNA library for the yeast two-hybrid system was constructed from <italic>B. pervariabilis × D. grandis</italic> shoots after 168 h treatment with <italic>A. phaeospermum</italic>. The library was identified as 1.20 × 10<sup>7</sup> cfu, with an average insert &gt;1,000 bp in size and a 100% positive rate, providing a database for the subsequent molecular study of the interaction between <italic>A. phaeospermum</italic> and <italic>B. pervariabilis × D. grandis</italic>. The yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC), and glutathione-S-transferase (GST) pull-down assays were used to screen for and identify two <italic>ApCtf1β</italic> interacting target proteins, <italic>BDUbc</italic> and <italic>BDSKL1</italic>, providing a reliable theoretical basis to study the molecular mechanism underlying <italic>B. pervariabilis × D. grandis</italic> resistance in response to <italic>A. phaeospermum</italic>, which would, in turn, establish a platform to develop new strategies for the sustainable and effective control of the blight diseases of forest trees.</p>